Hotspot

trnT-GGU-psbD

LSC · 31635-32883 · 1249 bp

Back to Paraphlomis

Pi 0.00353

MarkerSeek score 50.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1249
variable_sites: 30
pi: 0.00353
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1408-1435 bp	1.0	80.5
trnT-GGU-psbD_p2	ATAGTGACTCGTCCGGGAAT	GGACCAGCCTACAAAAACGA	50.0	50.0	57.9	58.1	1454-1481 bp	1.0	80.4
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1411-1438 bp	1.0	80.3
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1398-1425 bp	1.0	79.4
trnT-GGU-psbD_p5	ATAGTGACTCGTCCGGGAAT	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1444-1471 bp	1.0	79.3