Hotspot

trnH-GUG-psbA

LSC · 77-309 · 233 bp

Back to Paraphlomis

Pi 0.0271

MarkerSeek score 67.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 233
variable_sites: 33
pi: 0.0271
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	55.0	50.0	58.1	57.8	349-359 bp	1.0	85.8
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	ATCAACCGTGCTAACCTTGG	55.0	50.0	58.1	58.2	351-361 bp	1.0	85.7
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	362-372 bp	1.0	85.5
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	363-373 bp	1.0	85.4
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	364-374 bp	1.0	85.4