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rps8-rpl14

LSC · 82947-83181 · 235 bp

Back to Paraphalaenopsis

Pi 0.0171

MarkerSeek score 67.20

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 235
variable_sites: 6
pi: 0.0171
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	CGGCAATAATGTCCCTACCC	TGGTGCAATTGCTCGAGAAT	55.0	45.0	58.1	58.2	322-330 bp	1.0	81.2
rps8-rpl14_p2	CGGCAATAATGTCCCTACCC	ATTGCTCGAGAATTGCGACA	55.0	45.0	58.1	58.3	315-323 bp	1.0	80.4
rps8-rpl14_p3	CGGCAATAATGTCCCTACCC	TTGGTGCAATTGCTCGAGAA	55.0	45.0	58.1	58.4	323-331 bp	1.0	79.4
rps8-rpl14_p4	CGGCAATAATGTCCCTACCC	TTTGGTGCAATTGCTCGAGA	55.0	45.0	58.1	58.4	324-332 bp	1.0	79.4
rps8-rpl14_p5	CGGCAATAATGTCCCTACCC	TTTTGGTGCAATTGCTCGAG	55.0	45.0	58.1	57.6	325-333 bp	1.0	79.2