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rps11-rpl36

LSC · 81622-81974 · 353 bp

Back to Paraphalaenopsis

Pi 0.0867

MarkerSeek score 76.20

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 353
variable_sites: 32
pi: 0.0867
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	ACCAATACGTGCATTCTTACG	GACATAAACAGAGACAGGGGT	42.9	47.6	56.7	57.0	251-432 bp	1.0	56.8
rps11-rpl36_p2	ACCAATACGTGCATTCTTACG	GAGACATAAACAGAGACAGGGG	42.9	50.0	56.7	57.9	253-434 bp	1.0	55.6
rps11-rpl36_p3	ACCAATACGTGCATTCTTACG	CCGAGACATAAACAGAGACAGG	42.9	50.0	56.7	58.2	255-436 bp	1.0	54.8
rps11-rpl36_p4	ACCAATACGTGCATTCTTACG	CGAGACATAAACAGAGACAGGG	42.9	50.0	56.7	58.2	254-435 bp	1.0	54.8
rps11-rpl36_p5	ACCAATACGTGCATTCTTACG	ACATAAACAGAGACAGGGGT	42.9	45.0	56.7	55.7	250-431 bp	1.0	54.7