Hotspot

rbcL-accD

LSC · 56977-57795 · 819 bp

Back to Paraphalaenopsis

Pi 0.02

MarkerSeek score 67.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 819
variable_sites: 24
pi: 0.02
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CTAGCAAATGGAGCCCTGAG	CCACTGGTATGTCCAAGAGC	55.0	55.0	58.0	58.0	1009-1027 bp	1.0	82.8
rbcL-accD_p2	TGAAGCTAGCAAATGGAGCC	CCACTGGTATGTCCAAGAGC	50.0	55.0	58.2	58.0	1014-1032 bp	1.0	81.1
rbcL-accD_p3	TTTCGACCCAGTGGATAAGC	CCACTGGTATGTCCAAGAGC	50.0	55.0	57.6	58.0	948-966 bp	1.0	80.1
rbcL-accD_p4	GCTGCTTGTGAAGTCTGGAA	CCACTGGTATGTCCAAGAGC	50.0	55.0	58.4	58.0	983-1001 bp	1.0	79.9
rbcL-accD_p5	TCGTGAAGCTAGCAAATGGA	CCACTGGTATGTCCAAGAGC	45.0	55.0	57.2	58.0	1017-1035 bp	1.0	77.0