Hotspot

trnR-UCU-atpA

LSC · 10367-10529 · 163 bp

Back to Paraphalaenopsis

Pi 0.045

MarkerSeek score 77.10

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 163
variable_sites: 11
pi: 0.045
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGGGCGTCCATTGTCTAATG	AGCTGAAACCCTTTTGAGAGA	50.0	42.9	57.7	57.1	294-316 bp	1.0	69.0
trnR-UCU-atpA_p2	GAAGGGCGTCCATTGTCTAA	AGCTGAAACCCTTTTGAGAGA	50.0	42.9	57.6	57.1	296-318 bp	1.0	68.4
trnR-UCU-atpA_p3	ATAGGAATGAAGGGCGTCCA	AGCTGAAACCCTTTTGAGAGA	50.0	42.9	58.5	57.1	304-326 bp	1.0	67.5
trnR-UCU-atpA_p4	TAGGAATGAAGGGCGTCCAT	AGCTGAAACCCTTTTGAGAGA	50.0	42.9	58.5	57.1	303-325 bp	1.0	67.5
trnR-UCU-atpA_p5	AATGAAGGGCGTCCATTGTC	AGCTGAAACCCTTTTGAGAGA	50.0	42.9	58.5	57.1	299-321 bp	1.0	67.2