Hotspot

petD

LSC · 75331-76555 · 1225 bp

Back to Paraleonurus

Pi 0.00147
MarkerSeek score 52.30
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1225
variable_sites
4
pi
0.00147
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 AGTTGAAGGGAATTCTCCGA AGGGAAAATTCGCTTTGAAGC 45.0 42.9 56.2 57.7 1328 bp 1.0 55.1
petD_p2 TGAAGGGAATTCTCCGAAGAG AGGGAAAATTCGCTTTGAAGC 47.6 42.9 57.1 57.7 1325 bp 1.0 54.6
petD_p3 AGTTGAAGGGAATTCTCCGAAG AGGGAAAATTCGCTTTGAAGC 45.5 42.9 58.1 57.7 1328 bp 1.0 53.4
petD_p4 GTTGAAGGGAATTCTCCGAAGA AGGGAAAATTCGCTTTGAAGC 45.5 42.9 58.1 57.7 1327 bp 1.0 53.3
petD_p5 AGTTGAAGGGAATTCTCCGAA AGGGAAAATTCGCTTTGAAGC 42.9 42.9 56.9 57.7 1328 bp 1.0 53.3