Hotspot

petB

LSC · 73749-75136 · 1388 bp

Back to Paraleonurus

Pi 0

MarkerSeek score 51.60

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1388
variable_sites: 0
pi: 0
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TCACAAAGTCCTGGCTTTTGA	TCCTATTATTCCTCCTCTAAGTGA	42.9	37.5	58.0	55.6	1565-1568 bp	1.0	40.2
petB_p2	TCACAAAGTCCTGGCTTTTGA	TCCTCCTCTAAGTGATAAATGATTGA	42.9	34.6	58.0	57.4	1556-1559 bp	1.0	40.2
petB_p3	TCACAAAGTCCTGGCTTTTGA	TCCTCCTCTAAGTGATAAATGA	42.9	36.4	58.0	53.4	1556-1559 bp	1.0	40.2
petB_p4	TCACAAAGTCCTGGCTTTTGA	TCCATACTTGTAGCAATAAAATCCT	42.9	32.0	58.0	56.3	1586-1589 bp	1.0	40.2
petB_p5	TCACAAAGTCCTGGCTTTTGA	TTCCTCCTCTAAGTGATAAATGA	42.9	34.8	58.0	54.3	1557-1560 bp	1.0	40.2