Hotspot

atpF

LSC · 11625-12834 · 1210 bp

Back to Paraleonurus

Pi 0.00132

MarkerSeek score 52.70

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1210
variable_sites: 4
pi: 0.00132
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ATTTCGTCGGCTTGAATGGT	AAGAGGGACAAAGTGGTAGA	45.0	45.0	58.2	55.4	1385-1391 bp	1.0	58.1
atpF_p2	ATTTCGTCGGCTTGAATGGT	AGAGGGACAAAGTGGTAGAA	45.0	45.0	58.2	55.4	1384-1390 bp	1.0	58.1
atpF_p3	GTCGGCTTGAATGGTTACCA	AAGAGGGACAAAGTGGTAGA	50.0	45.0	58.2	55.4	1380-1386 bp	1.0	58.1
atpF_p4	GTCGGCTTGAATGGTTACCA	AGAGGGACAAAGTGGTAGAA	50.0	45.0	58.2	55.4	1379-1385 bp	1.0	58.1
atpF_p5	TCGTCGGCTTGAATGGTTAC	AGAGGGACAAAGTGGTAGAA	50.0	45.0	58.3	55.4	1381-1387 bp	1.0	57.3