Hotspot

accD

LSC · 56606-58072 · 1467 bp

Back to Paraleonurus

Pi 0.00164

MarkerSeek score 54.00

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1467
variable_sites: 6
pi: 0.00164
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ATAGGGGGCAAGAAAACTCT	TCCGCCACTTTTGATTCTTTC	45.0	42.9	56.1	57.1	1605 bp	1.0	49.7
accD_p2	ATAGGGGGCAAGAAAACTCT	CCGCCACTTTTGATTCTTTCT	45.0	42.9	56.1	57.1	1604 bp	1.0	49.7
accD_p3	AATAGGGGGCAAGAAAACTCT	TCCGCCACTTTTGATTCTTTC	42.9	42.9	56.9	57.1	1606 bp	1.0	48.0
accD_p4	AATAGGGGGCAAGAAAACTCT	CCGCCACTTTTGATTCTTTCT	42.9	42.9	56.9	57.1	1605 bp	1.0	48.0
accD_p5	ATAGGGGGCAAGAAAACTCT	TCCGCCACTTTTGATTCTTT	45.0	40.0	56.1	55.9	1605 bp	1.0	47.8