Hotspot

rps12-clpP

LSC · 68838-68975 · 138 bp

Back to Paraleonurus

Pi 0.00725

MarkerSeek score 63.20

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 138
variable_sites: 2
pi: 0.00725
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	TGACATTTCGAATGGGCTGT	TATGTCAGCAACAGAAGCCC	45.0	50.0	57.8	57.9	244-251 bp	1.0	80.4
rps12-clpP_p2	TGACATTTCGAATGGGCTGT	ACAAAGGACAGGCAAACCAT	45.0	45.0	57.8	57.9	301-308 bp	1.0	80.1
rps12-clpP_p3	TTTCGAATGGGCTGTCTTGT	TATGTCAGCAACAGAAGCCC	45.0	50.0	57.7	57.9	239-246 bp	1.0	79.9
rps12-clpP_p4	TTTCGAATGGGCTGTCTTGT	ACAAAGGACAGGCAAACCAT	45.0	45.0	57.7	57.9	296-303 bp	1.0	79.5
rps12-clpP_p5	ACATTTCGAATGGGCTGTCT	ACAAAGGACAGGCAAACCAT	45.0	45.0	57.5	57.9	299-306 bp	1.0	77.7