Hotspot

ycf1

SSC · 125216-130816 · 5601 bp

Back to Parakeelya

Pi 0.116

MarkerSeek score 77.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5601
variable_sites: 609
pi: 0.116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCTCTTCCACTCGTCGAAAT	AAAGAAACGGAAGAAGGCGT	50.0	45.0	58.0	58.0	714 bp	0.5	59.7
ycf1_p2	ATCTTCCGACCATTCTGCAC	AAAGAAACGGAAGAAGGCGT	50.0	45.0	58.0	58.0	696 bp	0.5	59.5
ycf1_p3	GCTCTTCCACTCGTCGAAAT	AGAAACGGAAGAAGGCGTAC	50.0	50.0	58.0	57.9	712 bp	0.5	59.4
ycf1_p4	ATCTTCCGACCATTCTGCAC	AGAAACGGAAGAAGGCGTAC	50.0	50.0	58.0	57.9	694 bp	0.5	59.2
ycf1_p5	GCTCTTCCACTCGTCGAAAT	TTGGGGGTTTAAGCTTCTCG	50.0	50.0	58.0	57.8	448 bp	0.5	58.4