Hotspot

clpP

LSC · 70891-72990 · 2100 bp

Back to Parakeelya

Pi 0.0948

MarkerSeek score 78.50

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2100
variable_sites: 196
pi: 0.0948
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TAACGTTCGCTACCAATGGG	AGTAGCAATACGCAATGGGG	50.0	50.0	58.0	58.0	2809-2981 bp	1.0	90.5
clpP_p2	TAACGTTCGCTACCAATGGG	GTAGCAATACGCAATGGGGA	50.0	50.0	58.0	58.0	2808-2980 bp	1.0	90.4
clpP_p3	CTAGTACATGTTCCTCGGCG	AGTAGCAATACGCAATGGGG	55.0	50.0	57.9	58.0	2577-2744 bp	1.0	89.9
clpP_p4	CTAGTACATGTTCCTCGGCG	GTAGCAATACGCAATGGGGA	55.0	50.0	57.9	58.0	2576-2743 bp	1.0	89.8
clpP_p5	TAACGTTCGCTACCAATGGG	ACGCAATGGGGAGTTGATAC	50.0	50.0	58.0	58.0	2972 bp	0.5	59.6