Hotspot

ndhF-rpl32

SSC · 113766-114469 · 704 bp

Back to Parakeelya

Pi 0.11

MarkerSeek score 79.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 704
variable_sites: 77
pi: 0.11
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCCAAAATCCCCTACACGAT	AACGCTGCCAAATATCCCTT	50.0	45.0	57.9	57.9	1372 bp	0.5	58.4
ndhF-rpl32_p2	GCCAAAATCCCCTACACGAT	ACGCTGCCAAATATCCCTTT	50.0	45.0	57.9	57.9	1371 bp	0.5	58.4
ndhF-rpl32_p3	GCCAAAATCCCCTACACGAT	GGCCGATTCCTGTTCTTCAA	50.0	50.0	57.9	58.2	1466 bp	0.5	58.1
ndhF-rpl32_p4	GCCCATATACGCCGAAGATT	AACGCTGCCAAATATCCCTT	50.0	45.0	57.9	57.9	930 bp	0.5	58.1
ndhF-rpl32_p5	GCCCATATACGCCGAAGATT	ACGCTGCCAAATATCCCTTT	50.0	45.0	57.9	57.9	929 bp	0.5	58.1