Hotspot

ndhC-trnV-UAC

LSC · 50573-51711 · 1139 bp

Back to Parakeelya

Pi 0.0771

MarkerSeek score 80.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1139
variable_sites: 85
pi: 0.0771
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGGTTCAGGCATTTGCTCAT	GTCAGAGCACATGGAACCAT	45.0	50.0	58.1	57.9	2027-2055 bp	1.0	84.5
ndhC-trnV-UAC_p2	TGGTTCAGGCATTTGCTCAT	TTCAGGTGCTCGTTTAGTCG	45.0	50.0	58.1	57.9	2328-2351 bp	1.0	84.4
ndhC-trnV-UAC_p3	CAATGCTCCTTTTCGCCATG	TTCAGGTGCTCGTTTAGTCG	50.0	50.0	58.1	57.9	1983 bp	0.5	58.9
ndhC-trnV-UAC_p4	TGGTTCAGGCATTTGCTCAT	AGTTCGGTTCGATTCAGGTG	45.0	50.0	58.1	57.9	2363 bp	0.5	58.8
ndhC-trnV-UAC_p5	CAATGCTCCTTTTCGCCATG	AGTTCGGTTCGATTCAGGTG	50.0	50.0	58.1	57.9	1995 bp	0.5	58.7