Hotspot

rps16-trnQ-UUG

LSC · 6127-6656 · 530 bp

Back to Parakeelya

Pi 0.076

MarkerSeek score 80.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 530
variable_sites: 40
pi: 0.076
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	603-628 bp	1.0	84.0
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	614-639 bp	1.0	83.4
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	45.0	50.0	58.1	58.2	633-658 bp	1.0	83.0
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	TAAGGCATCGGGTTTTGGTC	50.0	50.0	58.2	58.2	644-669 bp	1.0	82.4
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GTAAGGCATCGGGTTTTGGT	45.0	50.0	58.1	58.5	634-659 bp	1.0	80.7