Hotspot

trnK-UUU-rps16

LSC · 4295-4990 · 696 bp

Back to Parakeelya

Pi 0.0814
MarkerSeek score 83.30
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
696
variable_sites
55
pi
0.0814
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAGCCGAGTACTCTACCGTT AGAGGGCCGAGGTTTTTAAA 50.0 45.0 58.2 57.0 767-810 bp 1.0 78.6
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AGGGCCGAGGTTTTTAAAGA 50.0 45.0 58.2 57.0 765-808 bp 1.0 78.6
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT AAAGAGGGCCGAGGTTTTTA 50.0 45.0 58.2 57.0 769-812 bp 1.0 78.5
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AAGAGGGCCGAGGTTTTTAA 50.0 45.0 58.2 57.0 768-811 bp 1.0 78.5
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TAAAGAGGGCCGAGGTTTTT 50.0 45.0 58.2 57.0 770-813 bp 1.0 78.5