Hotspot

rpl14

LSC · 79670-80038 · 369 bp

Back to Paragynoxys

Pi 0

MarkerSeek score 26.30

Primer available yes

Label rpl14

Feature Metadata

label_name: rpl14
feature_type: gene
region: LSC
length_bp: 369
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14_p1	AACCATATCGCGGTCTCATT	TTCTTCGACCTTTGTATTGT	45.0	35.0	57.1	52.5	437 bp	1.0	40.0
rpl14_p2	ACCATATCGCGGTCTCATTT	TTCTTCGACCTTTGTATTGT	45.0	35.0	57.1	52.5	436 bp	1.0	40.0
rpl14_p3	AACCATATCGCGGTCTCATT	TTTCTTCGACCTTTGTATTGT	45.0	33.3	57.1	53.4	438 bp	1.0	40.0
rpl14_p4	ACCATATCGCGGTCTCATTT	TTTCTTCGACCTTTGTATTGT	45.0	33.3	57.1	53.4	437 bp	1.0	40.0
rpl14_p5	ACCATATCGCGGTCTCATTT	TTTTCTTCGACCTTTGTATTGT	45.0	31.8	57.1	54.2	438 bp	1.0	40.0