Hotspot

ndhH

SSC · 113019-114197 · 1179 bp

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Pi 0.00763

MarkerSeek score 53.70

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1179
variable_sites: 9
pi: 0.00763
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CGCTTTCAGCAATTCATGGA	GCATCCATATGATCCCATAGACC	45.0	47.8	57.1	58.3	1309 bp	1.0	46.8
ndhH_p2	TCGCTTTCAGCAATTCATGG	GCATCCATATGATCCCATAGACC	45.0	47.8	57.1	58.3	1310 bp	1.0	46.8
ndhH_p3	CGCTTTCAGCAATTCATGGA	TCCATATGATCCCATAGACCTCT	45.0	43.5	57.1	57.4	1306 bp	1.0	44.7
ndhH_p4	TCGCTTTCAGCAATTCATGG	TCCATATGATCCCATAGACCTCT	45.0	43.5	57.1	57.4	1307 bp	1.0	44.7
ndhH_p5	CGCTTTCAGCAATTCATGGAA	GCATCCATATGATCCCATAGACC	42.9	47.8	57.8	58.3	1309 bp	1.0	44.5