Hotspot

ndhD

SSC · 118955-120457 · 1503 bp

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Pi 0.00599

MarkerSeek score 62.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 9
pi: 0.00599
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CGCCAAAACCTGTGCTAAAA	TCATTAGCCAACCAACTCTT	45.0	40.0	57.5	54.5	1628-1631 bp	1.0	48.8
ndhD_p2	CGCCAAAACCTGTGCTAAAA	TTCATTAGCCAACCAACTCT	45.0	40.0	57.5	54.5	1629-1632 bp	1.0	48.8
ndhD_p3	GCACAGGTTTTTCTGGTCCA	TCATTAGCCAACCAACTCTT	50.0	40.0	58.6	54.5	1593-1596 bp	1.0	48.0
ndhD_p4	GCACAGGTTTTTCTGGTCCA	TTCATTAGCCAACCAACTCT	50.0	40.0	58.6	54.5	1594-1597 bp	1.0	48.0
ndhD_p5	CGCCAAAACCTGTGCTAAAA	TTCATTAGCCAACCAACTCTT	45.0	38.1	57.5	55.4	1629-1632 bp	1.0	47.6