Hotspot

trnL-TAG-rpl32

SSC · 121888-122691 · 804 bp

Back to Paragynoxys

Pi 0.00623
MarkerSeek score 62.30
Primer available yes
Label trnL-TAG-rpl32

Feature Metadata

label_name
trnL-TAG-rpl32
feature_type
igs
region
SSC
length_bp
804
variable_sites
5
pi
0.00623
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-TAG-rpl32_p1 AGCGTGTCTACCGATTTCAC GGCAGCGTTAAAAGCTTTGT 50.0 45.0 58.0 57.9 921-930 bp 1.0 79.3
trnL-TAG-rpl32_p2 CAGCGTGTCTACCGATTTCA GGCAGCGTTAAAAGCTTTGT 50.0 45.0 58.0 57.9 922-931 bp 1.0 79.3
trnL-TAG-rpl32_p3 AGCGTGTCTACCGATTTCAC AAAGGATATTGGGCAGCGTT 50.0 45.0 58.0 57.9 932-941 bp 1.0 79.2
trnL-TAG-rpl32_p4 CAGCGTGTCTACCGATTTCA AAAGGATATTGGGCAGCGTT 50.0 45.0 58.0 57.9 933-942 bp 1.0 79.2
trnL-TAG-rpl32_p5 TCCTAAGAGCAGCGTGTCTA GGCAGCGTTAAAAGCTTTGT 50.0 45.0 57.9 57.9 931-940 bp 1.0 78.4