Hotspot

rpl14-rpl16

LSC · 80039-80157 · 119 bp

Back to Paragynoxys

Pi 0.036

MarkerSeek score 62.40

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 119
variable_sites: 4
pi: 0.036
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	TCGACGATTGCTAGCTCCTA	GGTTACAGTAAGACCCGCAG	50.0	55.0	58.0	58.0	394-402 bp	1.0	82.3
rpl14-rpl16_p2	GCGTCTTTGATCACAGCAAC	GGTTACAGTAAGACCCGCAG	50.0	55.0	58.0	58.0	438-446 bp	1.0	82.0
rpl14-rpl16_p3	TCGACGATTGCTAGCTCCTA	ATACGCTCTTCAGGCACTTG	50.0	50.0	58.0	58.0	520-528 bp	1.0	81.6
rpl14-rpl16_p4	CGCACCAAAAACTCGAGTTC	GGTTACAGTAAGACCCGCAG	50.0	55.0	58.0	58.0	604-612 bp	1.0	81.5
rpl14-rpl16_p5	CGCACCAAAAACTCGAGTTC	ATACGCTCTTCAGGCACTTG	50.0	50.0	58.0	58.0	730-738 bp	1.0	81.1