Hotspot

rpl20-rps12

LSC · 67928-68669 · 742 bp

Back to Paragynoxys

Pi 0.0027

MarkerSeek score 60.30

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 742
variable_sites: 2
pi: 0.0027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCTCCGAGCGATATATCCCC	CAGCGTCGAGGAACATGTAC	55.0	55.0	57.9	58.7	808 bp	1.0	73.9
rpl20-rps12_p2	TCTCCGAGCGATATATCCCC	AGCGTCGAGGAACATGTACT	55.0	50.0	57.9	58.8	807 bp	1.0	73.2
rpl20-rps12_p3	TCTCCGAGCGATATATCCCC	TCAGCGTCGAGGAACATGTA	55.0	50.0	57.9	58.8	809 bp	1.0	73.1
rpl20-rps12_p4	TCTCCGAGCGATATATCCCC	GCGTCGAGGAACATGTACTA	55.0	50.0	57.9	57.2	806 bp	1.0	73.1
rpl20-rps12_p5	TTCTACGTCTCCGAGCGATA	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.8	58.7	815 bp	1.0	72.5