Hotspot

ndhC-trnV-TAC

LSC · 50140-50896 · 757 bp

Back to Paragynoxys

Pi 0.00396
MarkerSeek score 61.00
Primer available yes
Label ndhC-trnV-TAC

Feature Metadata

label_name
ndhC-trnV-TAC
feature_type
igs
region
LSC
length_bp
757
variable_sites
3
pi
0.00396
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-TAC_p1 CCCTTTGCTAATCGGAGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.1 57.8 901 bp 1.0 71.4
ndhC-trnV-TAC_p2 TCGGAGCTAAAAATCCCGAA AGAAGGTCTACGGTTCGAGT 45.0 50.0 56.9 57.8 890 bp 1.0 70.2
ndhC-trnV-TAC_p3 CCCTTTGCTAATCGGAGCTAA AGAAGGTCTACGGTTCGAGT 47.6 50.0 57.8 57.8 901 bp 1.0 69.1
ndhC-trnV-TAC_p4 CCCTTTGCTAATCGGAGCTA GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.1 57.5 900 bp 1.0 68.7
ndhC-trnV-TAC_p5 GGCCCTTTGCTAATCGGAG AGAAGGTCTACGGTTCGAGT 57.9 50.0 58.3 57.8 903 bp 1.0 68.4