Hotspot

trnT-TGT-trnL-TAA

LSC · 46137-46758 · 622 bp

Back to Paragynoxys

Pi 0.00666
MarkerSeek score 59.70
Primer available yes
Label trnT-TGT-trnL-TAA

Feature Metadata

label_name
trnT-TGT-trnL-TAA
feature_type
igs
region
LSC
length_bp
622
variable_sites
4
pi
0.00666
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-TGT-trnL-TAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 662-683 bp 1.0 79.9
trnT-TGT-trnL-TAA_p2 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 694-715 bp 1.0 78.9
trnT-TGT-trnL-TAA_p3 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 656-677 bp 1.0 78.7
trnT-TGT-trnL-TAA_p4 GGAATCGAACCGATGACCAT CGTCTACCGATTTCGCCATA 50.0 50.0 57.8 57.6 688-709 bp 1.0 77.7
trnT-TGT-trnL-TAA_p5 GCGATGCTCTAACCTCTGAG CGTAGCGTCTACCGATTTCG 55.0 55.0 57.9 58.6 661-682 bp 1.0 77.0