Hotspot

trnE-TTC-rpoB

LSC · 12195-12941 · 747 bp

Back to Paragynoxys

Pi 0.0027

MarkerSeek score 60.90

Primer available yes

Label trnE-TTC-rpoB

Feature Metadata

label_name: trnE-TTC-rpoB
feature_type: igs
region: LSC
length_bp: 747
variable_sites: 2
pi: 0.0027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-TTC-rpoB_p1	GGGAGTTGAACCCTCGTTTT	GTTCTTCTGTCAAACCCCGA	50.0	50.0	58.0	57.8	884-888 bp	1.0	78.4
trnE-TTC-rpoB_p2	GGGAGTTGAACCCTCGTTTT	AGTTCTTCTGTCAAACCCCG	50.0	50.0	58.0	57.7	885-889 bp	1.0	78.4
trnE-TTC-rpoB_p3	TAACCACTGGACCATAGGGG	GTTCTTCTGTCAAACCCCGA	55.0	50.0	58.1	57.8	843-847 bp	1.0	77.8
trnE-TTC-rpoB_p4	TAACCACTGGACCATAGGGG	AGTTCTTCTGTCAAACCCCG	55.0	50.0	58.1	57.7	844-848 bp	1.0	77.7
trnE-TTC-rpoB_p5	CCCTCGTTTTCTCCGTGAAA	GTTCTTCTGTCAAACCCCGA	50.0	50.0	58.1	57.8	874-878 bp	1.0	77.6