Hotspot

psbM-trnD-GTC

LSC · 11030-11672 · 643 bp

Back to Paragynoxys

Pi 0.00311
MarkerSeek score 60.50
Primer available yes
Label psbM-trnD-GTC

Feature Metadata

label_name
psbM-trnD-GTC
feature_type
igs
region
LSC
length_bp
643
variable_sites
2
pi
0.00311
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GTC_p1 GGAACTAAAATGAACAGTGCAGT TTCAATAGGCTAGAGCACCG 39.1 50.0 57.6 57.1 760-766 bp 1.0 45.6
psbM-trnD-GTC_p2 AAAATGAACAGTGCAGTAGC TTCAATAGGCTAGAGCACCG 40.0 50.0 54.5 57.1 754-760 bp 1.0 45.1
psbM-trnD-GTC_p3 ACTAAAATGAACAGTGCAGTAGC TTCAATAGGCTAGAGCACCG 39.1 50.0 57.4 57.1 757-763 bp 1.0 44.2
psbM-trnD-GTC_p4 GGAACTAAAATGAACAGTGCAGT CAATAGGCTAGAGCACCGC 39.1 57.9 57.6 58.1 758-764 bp 1.0 43.9
psbM-trnD-GTC_p5 AGGAACTAAAATGAACAGTGCAG TTCAATAGGCTAGAGCACCG 39.1 50.0 57.3 57.1 761-767 bp 1.0 43.5