Hotspot

ycf1

SSC · 107441-112447 · 5007 bp

Back to Paracalia

Pi 0.00499

MarkerSeek score 55.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5007
variable_sites: 25
pi: 0.00499
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATCCTTTTGGTCCTCCTCGA	TCCTTTTCCGTTTGAGTTTCAC	50.0	40.9	58.0	57.4	247 bp	1.0	60.2
ycf1_p2	ATCCTTTTGGTCCTCCTCGA	CCTTTTCCGTTTGAGTTTCACT	50.0	40.9	58.0	57.4	246 bp	1.0	60.2
ycf1_p3	CCGTTTTGGGAATGGAAACG	TCCTTTTCCGTTTGAGTTTCAC	50.0	40.9	57.9	57.4	271 bp	1.0	60.0
ycf1_p4	CGTTTTGGGAATGGAAACGG	TCCTTTTCCGTTTGAGTTTCAC	50.0	40.9	57.9	57.4	270 bp	1.0	60.0
ycf1_p5	CGTTTTGGGAATGGAAACGG	CCTTTTCCGTTTGAGTTTCACT	50.0	40.9	57.9	57.4	269 bp	1.0	60.0