Hotspot

trnV-GAC-ycf15

IRa · 135374-136053 · 680 bp

Back to Paracalia

Pi 0.00294

MarkerSeek score 60.40

Primer available yes

Label trnV-GAC-ycf15

Feature Metadata

label_name: trnV-GAC-ycf15
feature_type: igs
region: IRa
length_bp: 680
variable_sites: 2
pi: 0.00294
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-ycf15_p1	GATGACTTCCACCACGTCAA	ACCGTGAGAAAAGCAAGGAG	50.0	50.0	57.8	58.1	810 bp	1.0	78.0
trnV-GAC-ycf15_p2	GATGACTTCCACCACGTCAA	CGTGAGAAAAGCAAGGAGGT	50.0	50.0	57.8	58.1	808 bp	1.0	78.0
trnV-GAC-ycf15_p3	AACTCGCATTGGGTTTAGGG	ACCGTGAGAAAAGCAAGGAG	50.0	50.0	58.2	58.1	847 bp	1.0	77.9
trnV-GAC-ycf15_p4	AACTCGCATTGGGTTTAGGG	CGTGAGAAAAGCAAGGAGGT	50.0	50.0	58.2	58.1	845 bp	1.0	77.9
trnV-GAC-ycf15_p5	ATGACTTCCACCACGTCAAG	CGTGAGAAAAGCAAGGAGGT	50.0	50.0	57.8	58.1	807 bp	1.0	77.9