Hotspot

ycf15-trnV-GAC

IRb · 98145-98824 · 680 bp

Back to Paracalia

Pi 0.00294

MarkerSeek score 60.40

Primer available yes

Label ycf15-trnV-GAC

Feature Metadata

label_name: ycf15-trnV-GAC
feature_type: igs
region: IRb
length_bp: 680
variable_sites: 2
pi: 0.00294
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-trnV-GAC_p1	ACCGTGAGAAAAGCAAGGAG	GATGACTTCCACCACGTCAA	50.0	50.0	58.1	57.8	810 bp	1.0	78.0
ycf15-trnV-GAC_p2	CGTGAGAAAAGCAAGGAGGT	GATGACTTCCACCACGTCAA	50.0	50.0	58.1	57.8	808 bp	1.0	78.0
ycf15-trnV-GAC_p3	ACCGTGAGAAAAGCAAGGAG	AACTCGCATTGGGTTTAGGG	50.0	50.0	58.1	58.2	847 bp	1.0	77.9
ycf15-trnV-GAC_p4	CGTGAGAAAAGCAAGGAGGT	AACTCGCATTGGGTTTAGGG	50.0	50.0	58.1	58.2	845 bp	1.0	77.9
ycf15-trnV-GAC_p5	CGTGAGAAAAGCAAGGAGGT	ATGACTTCCACCACGTCAAG	50.0	50.0	58.1	57.8	807 bp	1.0	77.9