Hotspot

trnT-TGT-trnL-TAA

LSC · 46134-46756 · 623 bp

Back to Paracalia

Pi 0.0113

MarkerSeek score 65.40

Primer available yes

Label trnT-TGT-trnL-TAA

Feature Metadata

label_name: trnT-TGT-trnL-TAA
feature_type: igs
region: LSC
length_bp: 623
variable_sites: 7
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-TGT-trnL-TAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	684 bp	1.0	77.9
trnT-TGT-trnL-TAA_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	716 bp	1.0	77.0
trnT-TGT-trnL-TAA_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	678 bp	1.0	76.6
trnT-TGT-trnL-TAA_p4	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	50.0	50.0	57.8	57.6	710 bp	1.0	75.7
trnT-TGT-trnL-TAA_p5	GCGATGCTCTAACCTCTGAG	CGTAGCGTCTACCGATTTCG	55.0	55.0	57.9	58.6	683 bp	1.0	74.9