Hotspot

rpoC2-rps2

LSC · 23201-23421 · 221 bp

Back to Paracalia

Pi 0.0182

MarkerSeek score 63.40

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: LSC
length_bp: 221
variable_sites: 4
pi: 0.0182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	TTGGAGGGGGAAATGAAAGA	CCATGGCCAAAATGAACTCC	45.0	50.0	56.3	57.3	396-397 bp	1.0	62.0
rpoC2-rps2_p2	TTGGAGGGGGAAATGAAAGA	GGCCAAAATGAACTCCTGCT	45.0	50.0	56.3	58.7	392-393 bp	1.0	61.6
rpoC2-rps2_p3	TGGAGGGGGAAATGAAAGAT	CCATGGCCAAAATGAACTCC	45.0	50.0	56.1	57.3	395-396 bp	1.0	60.1
rpoC2-rps2_p4	TGGAGGGGGAAATGAAAGAT	GGCCAAAATGAACTCCTGCT	45.0	50.0	56.1	58.7	391-392 bp	1.0	59.6
rpoC2-rps2_p5	TTGGAGGGGGAAATGAAAGA	TGGCCAAAATGAACTCCTGC	45.0	50.0	56.3	59.0	393-394 bp	1.0	59.2