Hotspot

trnK-TTT-rps16

LSC · 4407-5241 · 835 bp

Back to Paracalia

Pi 0.0146

MarkerSeek score 68.80

Primer available yes

Label trnK-TTT-rps16

Feature Metadata

label_name: trnK-TTT-rps16
feature_type: igs
region: LSC
length_bp: 835
variable_sites: 12
pi: 0.0146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-TTT-rps16_p1	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	964-971 bp	1.0	78.4
trnK-TTT-rps16_p2	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	963-970 bp	1.0	78.4
trnK-TTT-rps16_p3	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.8	58.3	1049-1056 bp	1.0	77.7
trnK-TTT-rps16_p4	CAGTCGTGGTCTTCCAAACT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.8	58.3	1048-1055 bp	1.0	77.6
trnK-TTT-rps16_p5	TCCTAGCCGCACTTAAAAGC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.3	58.3	979-986 bp	1.0	77.6