Hotspot

rps15-ndhH

SSC · 115351-115447 · 97 bp

Back to Paraboea

Pi 0.0481

MarkerSeek score 71.90

Primer available yes

Label rps15-ndhH

Feature Metadata

label_name: rps15-ndhH
feature_type: igs
region: SSC
length_bp: 97
variable_sites: 14
pi: 0.0481
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ndhH_p1	GAAAACGTCAACGGCTTCTG	TCCCAACGCGTTACATAAGG	50.0	50.0	58.0	58.0	410 bp	0.285714	51.8
rps15-ndhH_p2	GAAAACGTCAACGGCTTCTG	AACACCATGCATTGATGGGT	50.0	45.0	58.0	58.1	273 bp	0.285714	51.7
rps15-ndhH_p3	GAAAACGTCAACGGCTTCTG	TTGATATTCAGCAACCCCGG	50.0	50.0	58.0	57.9	768 bp	0.285714	51.3
rps15-ndhH_p4	GAAAACGTCAACGGCTTCTG	GATATTCAGCAACCCCGGTT	50.0	50.0	58.0	57.9	766 bp	0.285714	51.3
rps15-ndhH_p5	GAAAACGTCAACGGCTTCTG	TCAGCAACCCCGGTTAAAAA	50.0	45.0	58.0	57.9	761 bp	0.285714	51.1