Hotspot

ycf1-rps15

SSC · 114725-115086 · 362 bp

Back to Paraboea

Pi 0.0419

MarkerSeek score 74.90

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 362
variable_sites: 37
pi: 0.0419
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	TTGCCTAGAAACGACGATGG	TCCCAACGCGTTACATAAGG	50.0	50.0	58.0	58.0	1451 bp	0.428571	58.4
ycf1-rps15_p2	TTGCCTAGAAACGACGATGG	CAGAAGCCGTTGACGTTTTC	50.0	50.0	58.0	58.0	1061 bp	0.428571	58.4
ycf1-rps15_p3	TTGCCTAGAAACGACGATGG	AACACCATGCATTGATGGGT	50.0	45.0	58.0	58.1	1314 bp	0.428571	58.2
ycf1-rps15_p4	GGTTGCCTAGAAACGACGAT	CAGAAGCCGTTGACGTTTTC	50.0	50.0	58.0	58.0	1063 bp	0.285714	52.7
ycf1-rps15_p5	GGTTGCCTAGAAACGACGAT	TCCCAACGCGTTACATAAGG	50.0	50.0	58.0	58.0	1453 bp	0.285714	52.6