Hotspot

psaJ-rpl33

LSC · 68037-68485 · 449 bp

Back to Paraboea

Pi 0.032

MarkerSeek score 71.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 449
variable_sites: 36
pi: 0.032
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CGAACTGAACTAAGAGCGCT	CGAACAAAACACCAATCCGG	50.0	50.0	58.0	58.0	1823-1849 bp	1.0	85.2
psaJ-rpl33_p2	GTTTTGGAGACCCACGTTCT	CGAACAAAACACCAATCCGG	50.0	50.0	58.0	58.0	1845-1871 bp	1.0	85.0
psaJ-rpl33_p3	CGAACTGAACTAAGAGCGCT	AAACACCAATCCGGGTTTGA	50.0	45.0	58.0	57.9	1817-1843 bp	0.857143	78.4
psaJ-rpl33_p4	GTTCTTGCAGTGAGAAACGC	CGAACAAAACACCAATCCGG	50.0	50.0	58.0	58.0	1528-1553 bp	0.714286	72.4
psaJ-rpl33_p5	GTTCTTGCAGTGAGAAACGC	AAACACCAATCCGGGTTTGA	50.0	45.0	58.0	57.9	1522-1547 bp	0.714286	72.2