Hotspot

petA-psbJ

LSC · 64105-65133 · 1029 bp

Back to Paraboea

Pi 0.0271

MarkerSeek score 70.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1029
variable_sites: 76
pi: 0.0271
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TTTATCCCGACGGAAGCAAG	ACAGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1967-1998 bp	1.0	85.5
petA-psbJ_p2	TTTATCCCGACGGAAGCAAG	AACAGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1968-1999 bp	1.0	85.4
petA-psbJ_p3	TTTATCCCGACGGAAGCAAG	CCGGTTTGGCTTACGATGTA	50.0	50.0	58.0	58.0	2047-2078 bp	1.0	85.2
petA-psbJ_p4	TTTATCCCGACGGAAGCAAG	TACCGGTTTGGCTTACGATG	50.0	50.0	58.0	58.0	2049-2080 bp	1.0	85.2
petA-psbJ_p5	TTTATCCCGACGGAAGCAAG	GTACCGGTTTGGCTTACGAT	50.0	50.0	58.0	58.0	2050-2081 bp	1.0	85.1