Hotspot

ycf4-cemA

LSC · 61416-62240 · 825 bp

Back to Paraboea

Pi 0.0257

MarkerSeek score 68.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 825
variable_sites: 55
pi: 0.0257
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GGGATCGCGGGTTTCTTTAT	GGAATTCCTGAGCCCAAGAG	50.0	55.0	58.0	58.0	1560-1706 bp	1.0	87.2
ycf4-cemA_p2	AATCGTCGCATATTCCTCCG	GGAATTCCTGAGCCCAAGAG	50.0	55.0	57.9	58.0	1431-1577 bp	1.0	87.1
ycf4-cemA_p3	CGTCGCATATTCCTCCGATT	GGAATTCCTGAGCCCAAGAG	50.0	55.0	57.9	58.0	1428-1574 bp	1.0	87.1
ycf4-cemA_p4	TGGTGCACAATTTCCTGGAA	GGAATTCCTGAGCCCAAGAG	45.0	55.0	57.9	58.0	1527-1673 bp	1.0	86.9
ycf4-cemA_p5	CGTTGGGGATTTCCTGGAAA	GGAATTCCTGAGCCCAAGAG	50.0	55.0	58.1	58.0	1452-1598 bp	1.0	86.9