Hotspot

ycf3-trnS-GGA

LSC · 44856-45739 · 884 bp

Back to Paraboea

Pi 0.0261

MarkerSeek score 69.40

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 884
variable_sites: 58
pi: 0.0261
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTATGGACTGAGCAGGGCTA	ATGGTTTCGACTATTCCCGC	50.0	50.0	57.8	58.1	2271-2297 bp	1.0	84.5
ycf3-trnS-GGA_p2	TTATGGACTGAGCAGGGCTA	CTGTATCATAGACCGGGGGT	50.0	55.0	57.8	58.0	1852-1870 bp	0.857143	78.3
ycf3-trnS-GGA_p3	ACTTCAACAGCCTTCGATGG	CTGTATCATAGACCGGGGGT	50.0	55.0	58.2	58.0	1699-1713 bp	0.714286	72.1
ycf3-trnS-GGA_p4	CTTCAACAGCCTTCGATGGT	CTGTATCATAGACCGGGGGT	50.0	55.0	58.2	58.0	1698-1712 bp	0.714286	72.1
ycf3-trnS-GGA_p5	CTGAGCAGGGCTAGTGTTTT	CTGTATCATAGACCGGGGGT	50.0	55.0	57.8	58.0	1845-1863 bp	0.714286	71.8