Hotspot

trnC-GCA-petN

LSC · 28349-29107 · 759 bp

Back to Paraboea

Pi 0.0207

MarkerSeek score 72.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 759
variable_sites: 43
pi: 0.0207
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	846-891 bp	1.0	87.2
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	50.0	50.0	58.1	58.0	844-889 bp	1.0	87.2
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	847-892 bp	1.0	86.6
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	792-837 bp	1.0	86.3
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	TCTAGAGCCCACTTCTTCCC	50.0	55.0	58.1	58.1	856-901 bp	1.0	86.2