Hotspot

atpH-atpI

LSC · 14038-15081 · 1044 bp

Back to Paraboea

Pi 0.0221

MarkerSeek score 71.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1044
variable_sites: 62
pi: 0.0221
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	AGTGGTTCCCATACCTGTCA	45.0	50.0	58.1	58.0	1180-1221 bp	1.0	85.0
atpH-atpI_p2	TCCGATAGAAGCAAGCCCTA	AGTGGTTCCCATACCTGTCA	50.0	50.0	57.9	58.0	1219-1260 bp	1.0	84.9
atpH-atpI_p3	TCCGATAGAAGCAAGCCCTA	TCAGTGGTTCCCATACCTGT	50.0	50.0	57.9	58.0	1221-1262 bp	1.0	84.9
atpH-atpI_p4	AACGGAAGCAGCAGAAATCA	TCAGTGGTTCCCATACCTGT	45.0	50.0	58.1	58.0	1182-1223 bp	1.0	84.9
atpH-atpI_p5	GATAGAAGCAAGCCCTACGG	AGTGGTTCCCATACCTGTCA	55.0	50.0	57.8	58.0	1216-1257 bp	1.0	84.4