Hotspot

atpF-atpH

LSC · 13387-13791 · 405 bp

Back to Paraboea

Pi 0.039
MarkerSeek score 74.80
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
405
variable_sites
38
pi
0.039
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.97
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 CCGTGCTGTGTCTAGACTTC TCCTTTCGTCTCGTTTCGAC 55.0 50.0 58.0 58.0 1325-1365 bp 1.0 86.8
atpF-atpH_p2 AAAAGGGTACAGCTGGTTGG GTCCTTTCGTCTCGTTTCGA 50.0 50.0 58.0 58.0 1140-1173 bp 0.714286 75.4
atpF-atpH_p3 AAAAGGGTACAGCTGGTTGG TCCTTTCGTCTCGTTTCGAC 50.0 50.0 58.0 58.0 1139-1172 bp 0.714286 75.4
atpF-atpH_p4 AAAAGGGTACAGCTGGTTGG AGTCCTTTCGTCTCGTTTCG 50.0 50.0 58.0 58.0 1141-1174 bp 0.714286 75.4
atpF-atpH_p5 AAAAGGGTACAGCTGGTTGG CCTTTCGTCTCGTTTCGACT 50.0 50.0 58.0 58.0 1138-1171 bp 0.714286 75.4