Hotspot

trnS-GCU-trnR-UCU

LSC · 8575-10372 · 1798 bp

Back to Paraboea

Pi 0.0288

MarkerSeek score 72.90

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1798
variable_sites: 124
pi: 0.0288
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	CAATCCGACGCTTTAGTCCA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	1720-1935 bp	1.0	72.9
trnS-GCU-trnR-UCU_p2	AATCCGACGCTTTAGTCCAC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	1719-1934 bp	1.0	72.9
trnS-GCU-trnR-UCU_p3	CAATCCGACGCTTTAGTCCA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	1726-1941 bp	1.0	72.2
trnS-GCU-trnR-UCU_p4	AATCCGACGCTTTAGTCCAC	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	1725-1940 bp	1.0	72.2
trnS-GCU-trnR-UCU_p5	AGCAATCCGACGCTTTAGTC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.4	56.9	1722-1937 bp	1.0	70.0