Hotspot

trnK-UUU-rps16

LSC · 4213-5028 · 816 bp

Back to Paraboea

Pi 0.0329

MarkerSeek score 76.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 816
variable_sites: 66
pi: 0.0329
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	ATACTTCCTTGAAAGGGGCG	50.0	50.0	58.2	57.6	939-949 bp	1.0	83.1
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	ATACTTCCTTGAAAGGGGCG	50.0	50.0	58.2	57.6	938-948 bp	1.0	83.1
trnK-UUU-rps16_p3	TCTAGCCGCACTTAAAAGCC	ATACTTCCTTGAAAGGGGCG	50.0	50.0	58.3	57.6	953-963 bp	1.0	82.2
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	ATACTTCCTTGAAAGGGGCG	50.0	50.0	58.3	57.6	948-958 bp	1.0	82.1
trnK-UUU-rps16_p5	TACCGTTGAGTTAGCAACCC	ATACTTCCTTGAAAGGGGCG	50.0	50.0	57.5	57.6	925-935 bp	1.0	80.9