Hotspot

trnH-GUG-psbA

LSC · 80-350 · 271 bp

Back to Paraboea

Pi 0.0926

MarkerSeek score 74.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 271
variable_sites: 49
pi: 0.0926
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	55.0	50.0	58.1	58.0	666-720 bp	1.0	89.0
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	CAGTGCTATGCATGGTTCCT	55.0	50.0	58.1	58.0	665-719 bp	1.0	89.0
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	680-734 bp	1.0	88.8
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	679-733 bp	1.0	88.8
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	681-735 bp	1.0	88.8