Hotspot

rps15

SSC · 116658-116933 · 276 bp

Back to Papilionanthe

Pi 0.0109

MarkerSeek score 45.00

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 276
variable_sites: 3
pi: 0.0109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	AAATAAGGAACTGCGTGGCA	CCCCAGACGTCGTAGAGAAT	45.0	55.0	58.1	58.6	1412-1414 bp	1.0	76.4
rps15_p2	CCGTTCATAAACAGCACAGC	CCCCAGACGTCGTAGAGAAT	50.0	55.0	57.8	58.6	1462-1464 bp	1.0	75.2
rps15_p3	CAAATAAGGAACTGCGTGGC	CCCCAGACGTCGTAGAGAAT	50.0	55.0	57.7	58.6	1413-1415 bp	1.0	75.1
rps15_p4	AAATAAGGAACTGCGTGGCA	CCCGGAATTTTCATGTTGTGG	45.0	47.6	58.1	58.0	1368-1370 bp	1.0	73.0
rps15_p5	AAATAAGGAACTGCGTGGCA	CCCCAGACGTCGTAGAGAA	45.0	57.9	58.1	58.1	1412-1414 bp	1.0	72.2