Hotspot

atpA

LSC · 11012-12535 · 1524 bp

Back to Papilionanthe

Pi 0.00394

MarkerSeek score 47.70

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 6
pi: 0.00394
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AGCCTTCCAAGCTAACGATG	CAAGCCTTACAACGAGCTCT	50.0	50.0	58.0	57.9	2104-2117 bp	1.0	80.6
atpA_p2	AGCCTTCCAAGCTAACGATG	AATTGAGCGTGAGAGCCAAA	50.0	45.0	58.0	58.1	2494-2507 bp	1.0	80.3
atpA_p3	CCTAGCCTTCCAAGCTAACG	CAAGCCTTACAACGAGCTCT	55.0	50.0	58.1	57.9	2107-2120 bp	1.0	80.3
atpA_p4	ACTAGAACCCTCGGCTTCTT	CAAGCCTTACAACGAGCTCT	50.0	50.0	58.1	57.9	2427-2440 bp	1.0	80.1
atpA_p5	AGCCTTCCAAGCTAACGATG	AGCCATTGAACAGCTCGAAA	50.0	45.0	58.0	58.1	2337 bp	0.5	58.9