Hotspot

rps15-ycf1

SSC · 116934-117397 · 464 bp

Back to Papilionanthe

Pi 0.0436

MarkerSeek score 68.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 464
variable_sites: 20
pi: 0.0436
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AAATAAGGAACTGCGTGGCA	CATTGCACGACACAACAACA	45.0	45.0	58.1	57.8	1490-1492 bp	1.0	79.7
rps15-ycf1_p2	CCGTTCATAAACAGCACAGC	CATTGCACGACACAACAACA	50.0	45.0	57.8	57.8	1540-1542 bp	1.0	78.5
rps15-ycf1_p3	CAAATAAGGAACTGCGTGGC	CATTGCACGACACAACAACA	50.0	45.0	57.7	57.8	1491-1493 bp	1.0	78.4
rps15-ycf1_p4	AAATAAGGAACTGCGTGGCA	ATCCATTGCACGACACAACA	45.0	45.0	58.1	58.4	1493-1495 bp	1.0	78.0
rps15-ycf1_p5	AAATAAGGAACTGCGTGGCA	CCATTGCACGACACAACAAC	45.0	50.0	58.1	58.5	1491-1493 bp	1.0	77.0