Hotspot

rpl16-rps3

LSC · 83537-84881 · 1345 bp

Back to Papilionanthe

Pi 0.0164

MarkerSeek score 55.60

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1345
variable_sites: 22
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	CCTCGGCAAGATATTCCCTT	TTTGGATATTTGTAAACCAAGAA	50.0	26.1	57.1	52.5	1445-1531 bp	1.0	41.3
rpl16-rps3_p2	GCCTCGGCAAGATATTCCC	TTTGGATATTTGTAAACCAAGAA	57.9	26.1	58.1	52.5	1446-1532 bp	1.0	41.3
rpl16-rps3_p3	CCTCGGCAAGATATTCCCTT	ATTTGGATATTTGTAAACCAAGA	50.0	26.1	57.1	52.3	1446-1532 bp	1.0	41.3
rpl16-rps3_p4	CCTCGGCAAGATATTCCCTT	TTGGATATTTGTAAACCAAGAAT	50.0	26.1	57.1	52.3	1444-1530 bp	1.0	41.3
rpl16-rps3_p5	CCTCGGCAAGATATTCCCTTC	TTTGGATATTTGTAAACCAAGAA	52.4	26.1	58.2	52.5	1445-1531 bp	1.0	41.3