Hotspot

rpl36-infA

LSC · 81542-81655 · 114 bp

Back to Papilionanthe

Pi 0.0268

MarkerSeek score 69.40

Primer available yes

Label rpl36-infA

Feature Metadata

label_name: rpl36-infA
feature_type: igs
region: LSC
length_bp: 114
variable_sites: 3
pi: 0.0268
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-infA_p1	GCCTACGAATCAAGCGACAT	TGATTCAACCAGGGGACGTA	50.0	50.0	58.4	58.4	228-230 bp	1.0	75.5
rpl36-infA_p2	TAACTATAATTCGCCCCCGC	TGATTCAACCAGGGGACGTA	50.0	50.0	57.5	58.4	246-248 bp	1.0	75.1
rpl36-infA_p3	GCCTACGAATCAAGCGACAT	CCGGCGCAGTTTTATACGTA	50.0	50.0	58.4	58.4	288-290 bp	1.0	74.4
rpl36-infA_p4	TAACTATAATTCGCCCCCGC	CCGGCGCAGTTTTATACGTA	50.0	50.0	57.5	58.4	306-308 bp	1.0	74.1
rpl36-infA_p5	GCCTACGAATCAAGCGACAT	ATCCGGCGCAGTTTTATACG	50.0	50.0	58.4	58.8	290-292 bp	1.0	71.7